Green Qpcr Primers

The primers are meticulously designed using origene s proprietary primer design algorithm developed from 10 000 qpcr experiments.

Green qpcr primers.

Sybr green for qpcr. The kit also includes a control primer set designed for amplification of the synthetic rna spike in provided in the mircury lna rt kit. To comply with miqe they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off target amplification 1. Sybr green i is a commonly used fluorescent dye that binds double stranded dna molecules by intercalating between the dna bases.

Our primer collection covers the entire human and mouse genomes. General description overview compatible with any thermal cycler kicqstart primers are ready to order pre designed primer pairs for quantifying gene expression by sybr green i rt qpcr reverse transcription quantitative real time pcr. Use of other reagents for qpcr will affect the results. Mark livingstone image 1 design qpcr primers image1 jpg image 2 design qpcr primers image2 page 1 jpg image 3 design qpcr primers image2 page 2 jpg.

The mircury lna mirna pcr assays and panels have been validated using the mircury sybr green master mix. Design sybr green qpcr primers experimental protocol oct 31 2013 recommendations. It is used in quantitative pcr because the fluorescence can be measured at the end of each amplification cycle to determine relatively or absolutely how much dna has been amplified. Refer to the mircury lna rt kit handbook for details.

Azuraquant green and probe fast qpcr master mixes for real time pcr real time pcr primer enzymes.