Other optical sectioning schemes like total internal reflection microscopy tirf or highly inclined laminated optical sheet microscopy hilo already became very successful in this field but because of their limited penetration depth they are not suitable for single molecule tracking in extended 3d samples. Highly inclined and laminated optical sheet hilo microscopy significantly improves the signal to background ratio by increasing the image intensity and decreasing the background intensity. Highly inclined and laminated optical sheet hilo microscopy is an optical technique that employs a highly inclined laser beam to illuminate the sample with a thin sheet of light that can be.
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